Serum lactate dehydrogenase isoenzyme (s) linked to immunoglobulin G with extremely decreased activity detected in myocardial infarct
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چکیده
LDH isoenzyme (s) linked to IgG of the lambda type found in the serum of a patient having had myocardial infarction is reported. This LDH-IgG complex has almost no enzymatic activity. It was found that the IgG of the patient acted as an inhibitor of LDH. LDH activity in normal human serum was inhibited and abnormal patterns of LDH isoenzyme (s) appeared when certain amounts of the patient's serum was added to a control serum as well as purified LDH isoenzymes. The IgG fraction in the serum was separated from the LDH-IgG complexes by 5'-AMP-Sepharose 4B column chromatography. Subclass analysis of IgG made it clear that the IgG in the serum was IgG3.
منابع مشابه
Aberrant creatine kinase and lactate dehydrogenase response in a myocardial infarct patient.
A 65-year-old woman failed to develop increased creatine kinase or lactate dehydrogenase activity after a myocardial infarction. She had no measurable creatine kinase MB isoenzyme and no detectable patterns of normal LD isoenzyme activity. These determinations five months after the infarction showed normal values for total activity and isoenzyme patterns.
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1. Tozawa T. Enzyme-linked immunoglobulins and their clinical significance. Electrophoresis 1989;10:640–4. 2. Wroblewski F, LaDue JS. Lactic dehydrogenase activity in blood. Proc Soc Exp Biol Med 1955;90:210–3. 3. Kanno T, Maekawa M, Uchiyama S, Tsutiya S. Criteria for detection of heterozygous individuals with lactate dehydrogenase subunit deficiencies. Clin Chem 1990;36:178–9. 4. Fujita K. De...
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Abnormal electrophoretic pattern of serum lactate dehydrogenase isoenzyme is shown. In this case electrophoretic pattern showed a sharp single band between lactate dehydrogenase 4 and lactate dehydrogenase 5 and broad band between lactate dehydrogenase 2 and lactate dehydrogenase 4. This abnormality was suggested to be caused by the presence of lactate dehydrogenase and immunoglobulin G (kappa ...
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تاریخ انتشار 2009